Samples, Services and Cost

SAMPLE TYPES

TIMC works with a broad range of samples encompassing many niches of the human microbiota. The microbiota refers to the microbial cells that live in a defined environment. Some of these sample types include:

• Environmental
• Oral
• Dental
• Nasal
• Stool
• Skin surface
• Biopsy tissue
• Formalin-Fixed Paraffin-Embedded (FFPE) preserved tissue

Such specimens enable us to study the many facets of the microbiome with our state-of-the-art sequencing technology and bioinformatics tools. Microbiome research has found multiple possible associations between various microbiota and diseases. Collection of samples related to these microbiota may provide further insight to clarify the prevalence of these associations.

SERVICES

We provide comprehensive microbiome services, including:

• Custom end-to-end service, including consultationing on study design, appropriate sample collection handling, and optimal analysis approaches
• Nucleic acid extraction
• Analysis-appropriate library preparation
• High-throughput sequencing
• Delivery of publication-quality bioinformatics analysis, statistics, and visualizations
• Post-analysis consultation on interpretation and future studies

Our sequencing applications for microbiome analysis include:

• Standard 16S (bacterial) gene sequencing
• Shallow and deep whole metagenome sequencing to understand full microbial gene composition
• Whole transcriptome sequencing to understand microbial and host transcription

The Center will assist with bioinformatics analysis and interpretation, including:

• Quality control and identification and removal of human genome contamination to protect your study participants’ identities
• Identification and annotation of amplicon sequence variants and other microbiome features
• Standard and customized microbiome analyses, including alpha and beta diversity analysis and visualization, differential abundance testing, longitudinal microbiome analysis, and more
• Generation of interactive visualizations that can be explored in your web browser
• Full annotation of all QIIME 2 analysis steps performed by our team, providing full transparency and enabling you to reproduce our workflow on the same or different microbiome data

Key data files will be provided, facilitating collaborator analysis with the QIIME 2 platform or other tools.

SEQUENCING COSTS

To provide an accurate quote under our new pricing system, we need detailed information about the exact services and the number of samples involved.

Please contact us at [email protected]. We can also return unused samples at an additional cost.