Users can provide samples for sequencing at multiple levels: from unprocessed tissue, isolated DNA or RNA, or premade platform compatible sequencing libraries. Depending on the submission scale, PCR-free human whole genome sequencing services with 30x analytical coverage (~107Gb) from isolated DNA can be as low as $510 today for batches in increments of 48 samples.
SEQUENCING
Short-read next-generation sequencing platforms
■ Illumina NovaSeq X Plus
- We stock 10B and 25B 300 cycle flow cells and sell individual lanes. Other flowcells can be ordered, but your request will need to use the entire flow cell.
- For the fastest turnaround we recommend 10B lanes.
- For most cost effective sequencing we recommend 25B lanes.
■ Illumina iSeq100
■ PacBio Onso
Long-read third generation sequencing platforms
■ Oxford Nanopore PromethION
- Includes up to three loadings over 72 hour run
■ PacBio Revio
- Options for 16, 24, or 30 hr movies.
SEQUENCING LIBRARY PREP
For both short read and long read sequencing applications we provide sequencing library preparation services for a variety of common applications.
Illumina Short Read
■ Genome, PCR-free or amplified
■ Exome/Target Capture
- Libraries can be created with adaptors containing inline Unique Molecular Identifiers (UMI) for high accuracy duplex sequencing or standard, non-UMI, adaptors
- We stock Twist Bioscience Exome 2.0
- Please contact us for custom capture needs
■ RNA, ribosomal depleted or mRNA
■ PacBio Onso Short Reads
■ Oxford Nanopore PromethION Long-reads
- Genome, Ultra-long (genome assembly)
- Genome, Native (unfragmented), typical N50 ~ 35kb
- Genome, fragmented (target lengths of 10kb or 20kb)
- Maximum yield per PromethION flow cell
■ PacBio Revio HiFi long reads
- Genome, fragmented SMRT-bell library (target lengths of 18-22kb)
- RNA, Kinnex Full Length RNA library
- 1-12 sample multiplexing, 4-plex recommended.
DNA, RNA, or DUAL DNA/RNA ISOLATIONS
- All isolations include full quality control
■ Nanodrop concentration, 260/280, 260/230
■ Fluorescent concentration (dsDNA or RNA)
■ Tapestation or Femtopulse as appropriate
